Which animation file format supports interactivity and multimedia content?

The FLASH file format is specifically designed to support interactivity and multimedia content, making it an ideal choice for animations that require user engagement. FLASH enables the incorporation of vector graphics, allowing for smoother animations. It supports audio and video elements, which enhances the overall multimedia experience. Furthermore, FLASH interacts with user inputs, such as mouse clicks and keyboard events, enabling complex interactions and dynamic storytelling in animated sequences.

In contrast, other options like Animated GIF, JPEG, and PNG are primarily image formats that do not support interactivity. Animated GIFs can create simple looping animations but lack advanced features such as user interactions or embedded multimedia elements. JPEG is a still image format that does not support animation or interactivity, and PNG is used for static images with transparency but likewise lacks any interactive capabilities. These characteristics underscore why FLASH stands out as the correct choice for supporting interactive multimedia content.